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KMID : 0545120000100020208
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 2 p.208 ~ p.214
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Isolation and Characterization of Chitosanase-Producing Microorganism,Aureobacterium Sp.YL,from Crab Shells
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Lee, Dong Mi
Lee, Ei Leen/Lee, Kang Man
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Abstract
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A bacterial strain producing extracellular chitosanase was isolated from crab shells and identified as a member of the genus Aureobacterium. The production of chitosanase was proportionally related to the microbial growth, induced by the presence of chitosan, and repressed by glucose at 0.5% (w/v) concentration or higher. The optimal culture conditions for the production of chitosanase were 30℃ and pH 7.0. Among the nitrogen sources tested, incubation with 0.25% (w/v) concentrations of tryptone and casitone showed the best production of chitosanase. The chitosanase of Aureobacterium sp. YL produced chitobiose as a major product and glucosamine, chitotriose, chitotetraose, and chitopentaose as minor products from chitosan.
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